Combat Sports in the Ancient World General Aspects of...

Following Poliakoff’s introduction to the ancient combat sports, he proceeds to provide a basic outline to the world of ancient combat sports in a series of sections within the chapter, including: The definition of a ‘combat sport’ and its relation to recreation and training; similarities amongst combat sports, training methods and common practise; the premise of athletic festivals, and the organization of said competition. The first area that Poliakoff studies is ‘General aspects of the ancient combat sports’ is the definition of a combat sport, which he splits into two explanations. Firstly, the idea of sport or athletics, Poliakoff believes, is an â€Å"activity in which a person physically competes against another in contest with†¦show more content†¦Combat sports and warfare for spectators are not necessarily mutually exclusive, and the opinions of the difference between them are variable on the opinion of the spectator or competitor. Furthermore, Guttmann suggests that â€Å"Poliakoff’s stipulation that the criteria for determining victory in sports must be â€Å"different from those that mark success in everyday life†...one can nevertheless assert that the rules...were sufficiently different from the rules of war...to allow us to distinguish the two forms of combat.† Combat sports in the modern world benefit much greater from this definition, considering the rules of modern warfare don’t hold much for hand-to-hand combat nor fencing or other forms thereof. However, a modern equivalent can possibly be found in Poliakoff’s reasoning in the difference between a martial art and a combat sport, where the move from sparring into competition distanced martial arts from their combat-oriented origins. 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The Arctic Circle Lies At The Northernmost Point Of The Earth

Greenland and Finland The Arctic Circle lies at the northernmost point of the Earth. Several countries have areas that are within the Arctic Circle. Those countries are Canada, United States of America, Russia, Greenland, Finland, Norway, Iceland, and Sweden. For decades this cold and snowy part of the word was a mystery to those outside of it. Within the last decade many television reality shows have brought the reality of this cold region of the world into clear focus for those of us that have never experienced it. Television shows such as â€Å"Deadliest Catch†, â€Å"Ice Road Truckers†, etc. Even though many of these countries share many similarities they also can be starkly different. Two countries within the Arctic Circle that†¦show more content†¦Greenland is comprised of 836,330 square miles of surface area. This is three times the size of Texas (Clark, 1943). The northwestern section of Finland is located on the Scandinavian Peninsula, while the reminder of the co untry lies in Europe. Finland only has a surface area of 130,559 square miles (â€Å"Finland in facts, 2016†). The area of Greenland is nearly six and a half times greater than that of Finland. With Greenland being three times the state of Texas, comparatively Kentucky is six and a half times smaller than Texas. The vastness of Greenland dwarfs the land mass of Finland. Another noteworthy lithospheric difference between the two countries lies in the tectonic differences. Greenland is located on a single tectonic plate with no fault lines within its borders. Therefore, Greenland has no volcanic activity whatsoever. Moreover, there is no evidence that it has had volcanic activity in millions of years. With this low amount of volcanic activity, Greenland possesses some of the most ancient rocks on the planet. These rocks have been left undisturbed, because of the lack of volcanic activity. With no fault lines Greenland should not be effected by earthquakes, however glacial earthquakes are quite powerful. These glacial earthquakes have been attributed to the effects of global warming on the glacial ice that sits atop the majority of Greenland’s surface. With the calving of the ice creating a gigaton, or a

Diploma of Counselling

Question: Discuss about the Diploma of Counselling. Answer: As a clinical psychologist, I conduct intake diagnostic interviews of the clients who present with their psychological problems. Assuming myself as a client, I can experience their feelings when they inform about their social history. While informing my family life, I will be quite comfortable to inform about my nuclear family and the relationship that I share with my parents and siblings. Although this is an easy step for me, it might not be so easy for the clients who do not open up easily and do not wish to talk about their family life. When asked about my extended family, I will take some time to think and pass over the necessary information. During my process of thinking, I will have to judge my relationship status that I share with my extended family and this process may include the careless actions like tapping my foot or playing with my hair (Nakash and Saguy 2015). If I try to hide something from the clinician, I will try to avoid making eye contact with him and act nervous (Nakash and Alegra 2013). Since I am a person with a large friend circle, I will be happy to give out the relevant information about my friends and will be quite excited while recalling the memories of good times or may get depressed while speaking of the bad memories. Social modelling has been a gradual change that occurred over the time and took place in entire Australia. I live in a nuclear family in Perth, but I can well remember the days of my childhood when my parents used to leave for work and me with my siblings used to enjoy the company of our grandparents owing to the nearness of my extended family. It was a popular method of childcare in those days and we used to be influenced by their stories and life experiences. Extended families had the advantage of sharing of responsibilities that has changed over time and formal childcare systems are now in place for most of the nuclear families (Klocker and Gibson 2013). As a kid, I was an avid follower and fan of sports and I used to regard the athletes as role models. I used to follow the sports activities and even went for live matches to the stadiums. However, with my growth and maturity, I was disappointed with the transgressions of the athletes for a period. Although I cannot rule out those influences of my role models as a kid but certainly, I can be aware of those influences from the point of view of a counsellor. Many of the athletes from different games used to influence me and I used to consider them as role models. Their aggression for victory and their precision in performance always made me dream to be an aspiring athlete and lead their life of glory. With the passing time, I realized they are artists with flaws as I discovered few of my favourite athletes charged under doping scandal (Grunseit et al. 2012). Although it was like a dream getting shattered but that identified the counsellor in me and made me realize what is acceptable and what is not. Sports are for enjoying the performance and not for admiring the life of an athlete. However, the modelling influences have an impact my everyday life as they taught me the aggression for victory and perseverance under pressure. References Grunseit, A.C., Macniven, R., Orr, R., Grassmayr, M., Kelly, B., Davies, D., Colagiuri, S. and Bauman, A.E. 2012, "Australian athletes' health behaviours and perceptions of role modelling and marketing of unhealthy products",Health Promotion Journal of Australia: Official Journal of Australian Association of Health Promotion Professionals,vol. 23, no. 1, pp. 63-69. Klocker, N. and Gibson, C. 2013, "Looking inwards: Extended family living as an urban consolidation alternative",Planning Theory Practice,vol. 14, no. 4, pp. 555. Nakash, O. and Alegra, M. 2013, "Examination of the Role of Implicit Clinical Judgments During the Mental Health Intake",Qualitative Health Research,vol. 23, no. 5, pp. 645-654. Nakash, O. and Saguy, T. 2015, "Social Identities of Clients and Therapists During the Mental Health Intake Predict Diagnostic Accuracy",Social Psychological and Personality Science,vol. 6, no. 6, pp. 710-717.

Research Proposal on Tax Evasion Essay Example

Research Proposal on Tax Evasion Essay Tax evasion is the activity which is practised by companies and individuals and is characterized with the evasion of paying taxes with the help of different means. Taxes are considered to be a very important and essential factor which maintains the existence of the state. Individuals, small firms, big companies and great international corporations have to pay taxes to support the life of the country and all its spheres. For example, education, health care, salaries, grants, the work of the police, fire brigades, the work of the power stations which provide people with energy and water – everything depends on taxes. From its side, the state provides people with the possibility to work, live, enjoy themselves, use the advantages of the civilization, live in peace and security. As a reward for these opportunities the state requires taxes. Taxes appeared already at the dawn of the human civilization when the first countries appeared. The monarchs provided their people with security and demanded payment for it in the form of taxes. Today many people and organizations try to evade taxes with the help of the illegal means to save more money for themselves. The most common method to evade taxes is to reduce the real profit of an individual or the whole company, because the sum of the tax depends directly on the profit of the company (the higher the profit, the higher the tax). Tax evasion is considered to be a serious crime and it is punished severely. We will write a custom essay sample on Research Proposal on Tax Evasion specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Research Proposal on Tax Evasion specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Research Proposal on Tax Evasion specifically for you FOR ONLY $16.38 $13.9/page Hire Writer Taxes play an important role for the development of a company, so if rich companies which work in the developing countries and report dishonestly low taxes, the process of economic grow will be extremely low, because there will be no finance supply into the local and national budget. If a student expects to prepare a successful research proposal, he should devote much time to the research of the topic and only then he can try to investigate the other points of the topic and suggest writing his own research paper on it. Before that, one should succeed in research proposal writing and prepare an interesting, informative and thought-provoking paper which will persuade the professor that the problem is worth researching. Every student often faces troubles while writing a research proposal, because the process requires special rules and standards. A free sample research proposal on tax evasion found in the Internet is quite a good piece of writing assistance, because it is written by an expert. Due to the experience and knowledge of the professional writers and their free sample research proposals on tax evasion one can learn how to format the paper well and how to organize it in the convincing manner. At EssayLib.com writing service you can order a custom research proposal on Tax Evasion topics. Your research paper proposal will be written from scratch. We hire top-rated PhD and Master’s writers only to provide students with professional research proposal help at affordable rates. Each customer will get a non-plagiarized paper with timely delivery. Just visit our website and fill in the order form with all proposal details: Enjoy our professional research proposal writing service!

Life and Work of Leonora Carrington, Activist and Artist

Life and Work of Leonora Carrington, Activist and Artist Leonora Carrington (April 6, 1917–May 25, 2011) was an English artist, novelist, and activist. She was part of the Surrealist movement of the 1930s and, after moving to Mexico City as an adult, became a founding member of Mexicos women’s liberation movement. Fast Facts: Leonora Carrington Known For: Surrealist artist and writerBorn: April 6, 1917 in Clayton Green, Clayton-le-Woods, United KingdomDied: May 25, 2011 in Mexico City, MexicoSpouse(s): Renato Leduc, Emericko WeiszChildren: Gabriel Weisz, Pablo WeiszNotable Quote: I didnt have time to be anyones muse... I was too busy rebelling against my family and learning to be an artist. Early Life Leonora Carrington was born in 1917 in Clayton Green, Chorley, Lancashire, England, to an Irish mother married to a wealthy Irish textile manufacturer. In a family of four children, she was the only daughter, alongside her three brothers. Although she was educated by excellent governesses and sent to good schools, she was expelled from two different schools for rebellious misbehavior. Eventually, Carrington was sent abroad to Florence, Italy, where she studied at Mrs. Penroses Academy of Art. When Carrington was ten, she first encountered Surrealist art in a gallery in Paris, which cemented her desire to pursue a career as an artist. Her father strongly disapproved, but her mother supported her. Although she was presented at court when she came of age, Carrington was mostly disinterested in the niceties of society. Newcomer to the Art World In 1935, Carrington attended the Chelsea School of Art in London for one year, but she then transferred to London’s Ozenfant Academy of Fine Arts (established by the French modernist Amà ©dà ©e Ozenfant), where she spent the next three years studying her craft. Her family was not openly opposed to her artistic pursuits, but by this point, they were not actively encouraging her either. Carringtons greatest champion and patron at this time was Edward James, the noted Surrealist poet and art patron. James bought many of her early paintings. Years later, he still supported her work, and he arranged a show for her work at  Pierre Matisses New York gallery in 1947. Relationship With Max Ernst At an exhibition in London in 1936, Carrington encountered the work of Max Ernst, a German-born Surrealist who was 26 years her senior. Ernst and Carrington met at a London party the following year and quickly became inseparable, both artistically and romantically. When they moved to Paris together, Ernst left his wife and moved in with Carrington, making a home in the south of France. Together, they supported each other’s art and even made works of art, such as quirky animal sculptures, to decorate their shared home. It was during this period that Carrington painted her first clearly Surrealist work, Self-portrait  (also called  The Inn of the Dawn Horse). Carrington depicted herself in dreamy white clothes and with loose hair, with a prancing hyena in front of her a rocking horse flying around behind her. She also painted a portrait of Ernst in a similar style. When World War II began, Ernst (who was German) was immediately treated with hostility in France. He was soon arrested by French authorities as a hostile foreign national and was released only because of interventions of several well-connected French and American friends. Things only got worse when the Nazis invaded France; they arrested Ernst again and accused him of creating â€Å"degenerate† art. Ernst escaped and fled to America with the help of art patron Peggy Guggenheim- but he left Carrington behind. Ernst married Peggy Guggenheim in 1941, and although their marriage soon fell apart, he and Carrington never rekindled their relationship. Institutionalization and Escape Terrified and devastated, Carrington fled Paris and headed to Spain. Her mental and emotional state deteriorated, and ultimately her parents had Carrington institutionalized. Carrington was treated with electroshock therapy and strong drugs. Carrington later wrote about her horrific experiences in the mental institution, which also reportedly included assault, abuse, and unsanitary conditions, in a novel, Down Below. Eventually, Carrington was released to the care of a nurse and moved to Lisbon, Portugal. In Lisbon, Carrington escaped the nurse and sought sanctuary in the Mexican embassy. Renato Leduc, a Mexican ambassador and friend of Pablo Picasso, agreed to help get Carrington out of Europe. The pair entered a marriage of convenience so that her path would be smoother as a diplomat’s wife, and they were able to escape to Mexico. Aside from a few journeys north to the United States, Carrington would spend most of the rest of her life in Mexico. Art and Activism in Mexico Carrington and Leduc divorced quickly and quietly in 1943. Over the next couple of decades, Carrington spent time in New York City as well as in Mexico, interacting with the art world at large. Her work was unusual among the Surrealist community in that she did not use the works of Freud as a major influence. Instead, she utilized magical realism and the idea of alchemy, often drawing on her own life for inspiration and symbolism. Carrington also went against the grain with regards to the Surrealists’ approach to female sexuality: she painted as she experienced the world as a woman, rather than the male-gaze filtered depictions of many of her counterparts. In the 1970s, Leonora became a voice for the women’s liberation movement in Mexico City. She designed a poster, called Mujeres conciencia, for their movement. In many ways, her art tackled concepts of gender identity and feminism, making her an ideal fit to work with their cause. Her focus was psychological freedom, but her work was primarily towards political freedom for women (as a means to this ultimate goal); she also believed in creating cooperative efforts between the movements in North America and Mexico. While Carrington was living in Mexico, she met and married the Hungarian-born photographer Emerico Weisz. The couple had two sons: Gabriel and Pablo, the latter of whom followed in his mother’s footsteps as a Surrealist artist. Death and Legacy Carringtons husband Emerico Weisz died in 2007. She survived him by about four years. After a battle with pneumonia, Carrington died in Mexico City on May 25, 2011, aged 94. Her work continues to be shown at exhibitions across the world, from Mexico to New York to her native Britain. In 2013, Carringtons work had a major retrospective at the Irish Museum of Modern Art in Dublin, and in 2015, a Google Doodle commemorated what would have been her 98th birthday. By the time of her death, Leonora Carrington was one of the last-surviving Surrealist artists, and undoubtedly one of the most unique. Sources Aberth, Susan. Leonora Carrington: Surrealism, Alchemy and Art. Lund Humphries, 2010.Blumberg, Naomi. â€Å"Leonora Carrington: English-Born Mexican Painter and Sculptor.† Encyclopaedia Britannica, https://www.britannica.com/biography/Leonora-Carrington.â€Å"Leonora Carrington.† National Museum of Women in the Arts, https://nmwa.org/explore/artist-profiles/leonora-carrington.

Biography of Konrad Zuse, Inventor of Modern Computers

Biography of Konrad Zuse, Inventor of Modern Computers Konrad Zuse (June 22, 1910–December 18, 1995) earned the semi-official title of inventor of the modern computer for his series of automatic calculators, which he invented to help with his lengthy engineering calculations. Zuse  modestly dismissed the title, though, praising the inventions of his contemporaries and successors as being equally- if not more- important than his own. Fast Facts: Konrad Zuse Known For: Inventor of the first electronic, fully programmable digital computers, and a programming languageBorn: June 22, 1910 in Berlin-Wilmersdorf, GermanyParents: Emil Wilhelm Albert Zuse and Maria Crohn ZuseDied: December 18, 1995 in Hà ¼nfeld (near Fulda), GermanySpouse: Gisela Ruth BrandesChildren: Horst, Klaus Peter, Monika, Hannelore Birgit, and Friedrich Zuse Early Life Konrad Zuse was born on June 22, 1910, in Berlin-Wilmersdorf, Germany, and was the second of the two children of the Prussian civil servant and postal officer Emil Wilhelm Albert Zuse and his wife Maria Crohn Zuse. Konrads sister was named Lieselotte. He attended a series of grammar schools and briefly considered a career in art, but he eventually enrolled at the  Technical College (Technischen Hochschule) in Berlin-Charlottenburg, graduating with a degree in civil engineering in 1935. After graduation, he started work as a design engineer at the Henschel Flugzeugwerke (Henschel aircraft factory) in Berlin-Schà ¶nefeld. He resigned a year later after deciding to devote his life entirely to the construction of a computer, work that he pursued relentlessly between 1936 and 1964. The Z1 Calculator   One of the most difficult aspects of performing large calculations with slide rules or mechanical adding machines is keeping track of all the intermediate results and using them in their proper place during the later steps of the calculation. Zuse wanted to overcome that difficulty.  He realized that an automatic calculator would require three basic elements: a control, a memory, and a calculator for the arithmetic. Zuse made a mechanical calculator called the Z1 in 1936. This was the first binary computer. He used it to explore several groundbreaking technologies in calculator development: floating-point arithmetic, high-capacity memory, and modules or relays operating on the yes/no principle.   Electronic, Fully Programmable Digital Computers Zuses ideas were not fully implemented in the Z1  but they succeeded more with each Z prototype. Zuse completed the Z2,  the first fully functioning electro-mechanical computer in 1939,  and the Z3 in 1941. The Z3 used recycled materials donated by fellow university staff and students. It was the worlds first electronic, fully programmable digital computer based on a binary floating-point number and a  switching system. Zuse used old movie film to store his programs and data for the Z3 instead of paper tape or punched cards. Paper was in short supply in Germany during the war. According to The Life and Work of Konrad Zuse by Horst Zuse: In 1941, the Z3 contained almost all the features of a modern computer as defined by John von Neumann and his colleagues in 1946. The only exception was the ability to store the program in the memory together with the data. Konrad Zuse did not implement this feature in the Z3 because his 64-word memory was too small to support this mode of operation. Due to the fact that he wanted to calculate thousands of instructions in a meaningful order, he only used the memory to store values or numbers. The block structure of the Z3 is very similar to a modern computer. The Z3 consisted of separate units, such as a punch tape reader, control unit, floating-point arithmetic unit, and input/output devices.† Marriage and Family In 1945, Zuse married one of his employees, Gisela Ruth Brandes. They had five children: Horst, Klaus Peter, Monika, Hannelore Birgit, and Friedrich Zuse. The First Algorithmic Programming Language Zuse wrote the first algorithmic programming language in 1946. He called it Plankalkà ¼l and used it to program his computers. He wrote the worlds first chess-playing program using Plankalkà ¼l. The Plankalkà ¼l language included arrays and records and used a style of assignment- storing the value of an expression in a variable- in which the new value appears in the right column. An array is a collection of identically typed data items distinguished by their indices or subscripts,† such as  A[i,j,k], in which A is the array name and ​i, j, and k are the indices. Arrays are best when accessed in an unpredictable order. This is in contrast to lists, which are best when accessed sequentially. World War II Zuse was unable to convince the Nazi government to support his work for a computer based on electronic valves. The Germans thought they were close to winning the war and felt no need to support further research. The Z1 through Z3 models were shuttered, along with Zuse Apparatebau, the first computer company that Zuse formed in 1940. Zuse left for Zurich to finish his work on the Z4, which he smuggled from Germany in a military truck by hiding it in stables en route to Switzerland. He completed and installed the Z4 in the Applied Mathematics Division of Zurichs Federal Polytechnical Institute, where it remained in use until 1955.   The Z4 had a mechanical memory with a capacity of 1,024 words and several card readers. Zuse no longer had to use movie film to store programs since  he could now use punch cards. The Z4 had punches and various facilities to enable flexible programming, including address translation and conditional branching.   Zuse moved back to Germany in 1949 to form a second company called Zuse KG for the construction and marketing of his designs. Zuse rebuilt models of the Z3 in 1960 and the Z1 in 1984. Death and Legacy Konrad Zuse died on December 18, 1995, of a heart attack, in Hà ¼nfeld, Germany.  His innovations of fully working programmable calculators and a language to run it have established him as one of the innovators leading to the computing industry. Sources Dalakov, Georgi. Biography of Konrad Zuse. History of Computers.  1999.Zuse, Horst. Konrad Zuse- Biography. Konrad Zuse Homepage.  2013.Zuse, Konrad. The Computer, My Life. Trans. McKenna, Patricia and J. Andrew Ross. Heidelberg, Germany: Springer-Verlag, 1993.

Batting Biofilms Assignment Example | Topics and Well Written Essays - 500 words

Batting Biofilms - Assignment Example If genes which are responsible for the formation of these adhering protein molecules are deleted the bacteria are unable to form biofilms. However, it is now proved that bacteria form biofilms after communicating with each other through signal molecules, for e.g. in P. aeruginosa the relevant signaling molecules are acylated homoserine lactones which each cell produces at a low level, when enough cells assemble the concentration of these compounds increases which in turn trigger the activation of certain genes, the mechanism is known as quorum sensing, which is critical for the formation of biofilms. Since biofilms formed by P. aeruginosa is responsible for causing cystic fibrosis pneumonia, i.e. they turn virulent (disease causing). algC gene is responsible to synthesize alginate, the gelatinous polymer that makes extracellular matrix. Thus, signaling molecules to control the genes that code for virulence. During stage 1 when the bacterial cells move or swim freely and arrange themselves in cluster in order to form a biofilm; Attack strategy is employed that coats the molecules and block or disrupt microbial arrangement or attachment. During stage 2- when the collected cells begin producing a gooey matrix; Attack strategy is employed which coat the surfaces with substances that interfere with the matrix production so that film is not formed. During stage 4- when chemical gradient arise and promote the coexistence of diverse species and metabolic states; Attack strategy is employed which delivers multiple antibiotics or disinfectants to undermine the varied survival strategies of biofilm cells. Microbiologists took a very long time to size up the microbes in the biofilm. Since the establishment of germ theory, in the late 19th century by Robert Koch, bacteria were envisioned as single cells that float or swim through some kind of watery

The Effects of Baby Boomers Retiring Will Have on the Economy Research Paper

The Effects of Baby Boomers Retiring Will Have on the Economy - Research Paper Example At each stage, of its life cycle, the baby boom generation has changed the market for many products, altered the demand for public services, as well as the nature of the labor force. This paper delves into the effects the baby boomers’ retiring will have on the economy of the United States over the next 20 years. Introduction In the world’s history, baby boomers (who represent 28% of all the United States’ adult population) have been one of the most productive generations. This generation took advantage of subsidized scholarships and school loans from the government, which enabled them to get proper education, which in turn helped them to secure high paying jobs in all sectors of the US economy with tremendous added gains from globalization and newer technologies (Talbott, 2010). Social scientists, analysts, and policymakers have developed a great interest regarding the effects of the retirement of baby boomers on the economy of the US and the nation at large. Th e year 2005 marked the commencement of the exodus of baby boomers from the labor force. Since then, every seven seconds, a baby boomer attains the retirement age of sixty years, and this process will continue for the next twenty years. These retirements foreshadow a diminution of workplace knowledge as well as knowledge-based experience at a time when such experience and knowledge are more and more vital to the economy of the US as well as to the organizations that comprise it (Beazley, Boenich, and Harden, 2002). Estimates by the Employment Policy Foundation indicate that with baby boomers reaching the age of retirement, by the year 2012, businesses will experience a severe shortage of six million employees, and this number will increase to thirty-five million employees by the year 2030. Slower workforce growth implies that there will be slower rate of growth of the economy, and consequently, the living standards of everybody in the nation will be lower. Essentially, the baby boom generation’s retirement threatens to limit the potential of the economy of the United States, reducing the speed limit on how fast it can grow. Economists projects a considerable decline in the growth of the US economy to 2.2% by the year 2015, compared with a typical growth of about 3.2% during the last forty years (The special committee on aging, 2007). According to Gordon (2005), over the next next twenty years, a great number of baby boomers, roughly seventy million baby boomers, some highly skilled, will start leaving the job market of the United States of America gradually, with only forty million employees coming in. Following this retirement, skill shortages throughout the whole economy will get to critical levels. Woodruff (2011) points out that reports from the Pew Research Center indicate that for the next twenty years, over ten thousand baby boomers will be retiring daily. This retirement will have a remarkable effect on everybody and on all measures of the United States’ economic output (Talbott, 2010). For instance, it will lead to a slowdown of about 0.5% in the growth rate of in the workforce every year from its average 1.6% per annum since 1950-2007. The decline in the growth of the workforce in the United States is an indication that the nation’s labor supply may be inadequate to sustain the standards of living (Jarvik, 1980). The special committee on aging (2007) reports the fact that the aging and retirement of baby boomers will have potential impacts on the economy of th

The Dna Extraction Essay Example for Free

The Dna Extraction Essay 1. The salt contributes positively charged atoms that neutralise the normal negative charge of DNA. Salt is used at a high molarity due to the fact that it precipitates all of the proteins out. DNA is insoluble in low molar salt solutions but soluble in low molar salt solutions thus keeping the DNA in solution. 2. blending the onion will homogenize the mixture and it helps with the breakdown of the cell walls. Blending saves one the time and effort of using a motar and pestle, however it may break a lot of the DNA which is not favourable as one needs a lot of DNA for the extraction. 3. The enzymes in the soap are used to break down the lipid (fat) molecules of the cells nuclear membranes releasing the contents of the cell crucially including the DNA. These enzymes in the soap are what break down grease while washing dishes. 4. The DNA does not dissolve in this alcohol but rather pushes up through and out of the solution or precipitates. It is less dense than water or cell scum which is what settles to the bottom of the glass so it floats up into the alcohol layer, where you see it as a snotty, string-like substance, with small bubbles formed on it. 5. Because protein is stored in them for the nutrition of the new plants. 6. It is too small to be seen with the naked eye. What you extracted is millions of strands of DNA. In addition to that, whilst the substance was heated, the DNA got denatured which results in it looking more like a ladder than a helix. 7. Most of the DNA extracted during this proccess comes from the nucleus of the cell. AIM: The purpose of this experiment is to extract DNA from a variety of cells (Onion cells in particular) and see DNA molecules. This will show that, contrary to popular opinion, DNA is not just found in blood cells, but in a variety of tissues. Prior knowledge should include the fact that cell membranes are layers of lipids, or fat molecules, that DNA is found in the nucleus of a cell, and that enzymes speed up chemical reactions. HYPOTHESIS: DNA is present in the cells of all living organisms. METHOD: 1. Prepare two water baths one at 60Â °C and another filled with ice and water, around 4Â °C. For the hot water bath, a large metal pot can be used along with a thermometer with an appropriate temperature range. For the ice bath, a mixing bowl filled with ice and water works well. 2. For each onion, make a solution consisting of 10 ml of liquid dishwashing detergent and 1.5 g of table salt. Put in a 250 ml beaker and emulsify. 3. Add distilled water to make a final volume of 100 ml. Dissolve the salt by stirring slowly to avoid foaming. 4. Coarsely chop one large onion with a food processor or blender and put into a 1000 ml mixing bowl. For best results, do not chop the onion too finely. The size of the pieces should be like those used in making spaghetti. It is better to have the pieces too large than too small. 5. Cover chopped onion with the 100 ml of solution from step 2. The liquid detergent causes the cell membrane to break down and dissolves the lipids and proteins of the cell by disrupting the bonds that hold the cell membrane together. The detergent causes lipids and proteins to precipitate out of the solution. Salt enables nucleic acids to precipitate out of an alcohol solution because it shields the negative phosphate end of DNA, causing the DNA strands to come closer together and coalesce. 6. Put the measuring cup in a hot water bath at 60Â °C for 10-12 minutes. During this time, press the chopped onion mixture against the side of the measuring cup with the back of the spoon. (Do not keep the mixture in the hot water bath for more than 15 minutes because the DNA will begin to break down.) If using a large metal pot for water bath, remove the pot from the stove before placing the onion-containing measuring cup inside—the procedure is safer if the pot is off the burner. Cont inue to monitor temperature of water bath and make adjustments as needed. 7. The heat treatment softens the phospholipids in the cell membrane and denatures the DNAse enzymes which, if present, would cut the DNA into small fragments so that it could not be extracted. 8. Cool the mixture in an ice water bath for 5 minutes. During this time, press the chopped onion mixture against the side of the measuring cup with the back of the spoon. This step slows the breakdown of DNA. 9. Filter the mixture through a #6 coffee filter or four layers of cheese cloth placed in a strainer over a 4-cup measuring cup. When you filter the onion mixture, try to keep the foam from getting into the filtrate. It sometimes filters slowly, so you might want to put the whole set up in the refrigerator and let it filter overnight. 10. Dispense the onion solution into a test tube. The test tube should contain about 1 teaspoon of solution or be about 1/3 full. For most uniform results among test tubes, stir the solution frequently when dispensing it into the tubes. There is not an adva ntage to dispensing more than one teaspoon of solution into a test tube. The solution can be stored in a refrigerator for about a day before it is used for the laboratory exercise. When the solution is removed from the refrigerator, it should be gently mixed before the test tubes are filled. 11. Add cold alcohol to the test tube to create an alcohol layer on top of about 1 cm. For best results, the alcohol should be as cold as possible. The alcohol can be added to the solution in at least three ways: (a) Fill a pasteur pipette with alcohol, put it to bottom of the test tube, and release the alcohol. (b) Or, put about 1 cm of alcohol into the bottom of a test tube and add the onion solution. (c) Or, slowly pour the alcohol down the inside of the test tube with a pasteur pipette or medicine dropper. DNA is not soluble in alcohol. When alcohol is added to the mixture, all the components of the mixture, except for DNA, stay in solution while the DNA precipitates out into the alcohol layer. 1 2. Let the solution sit for 2-3 minutes without disturbing it. It is important NOT to shake the test tube. You can watch the white DNA precipitate out into the alcohol layer. When good results are obtained, there will be enough DNA to spool on to a glass rod, a pasteur pipette that has been heated at the tip to form a hook, or similar device. A wooden skewer or nut pick (small metal rod with curved tip) may also work well for spooling DNA if Pasteur pipette is unavailable. DNA has the appearance of white mucus. CONCLUSION: With these findings we can conclude that DNA is present and can be found in the cells of all living organisms and not just in those of the human body.

Hamlet: Moral Order :: Shakespeare Hamlet Essays

Hamlet: Moral Order In Shakespeare's Hamlet, a very clear moral order is established as the protagonist, Hamlet, completes his journey through the phases which define a Shakespearean tragedy. The play begins with Hamlet encountering his father's ghost, at which point he learns his father had in fact been murdered by his own brother, Claudius. It is Hamlet's wish to avenge his father that causes all other moral dilemmas in the play, and this is what defines the play's particular moral order: As the play progresses, the gravity and seriousness of Claudius sins lessen, and Hamlet's grow, although never reaching the moral plateau on which Claudius rests. In the beginning of the play, Hamlet is morally "in the right", always taking precautions to ensure this remains so. Claudius, on the other hand, not only murders Hamlet's father, but then plots to do away with Hamlet as soon as he feels threatened. As the play progresses, Hamlet continues attempting to right the original wrong, but only succeeds at the finish, with Claudius' death. Hamlet's words in Act III, Scene IV -- "thus bad begins, and worse remains behind" illustrate the moral order well; the actions against him were wrong, but, to a lesser extent, so was his revenge. Near the start of the play, The Ghost tells Hamlet of the crime committed by Claudius. When Hamlet finds out his father was murdered by his own brother, who then stole his wife and crown, he immediately commits himself to avenging the murder; "Haste me to know't, that I, with wings as swift/As meditation or the thoughts of love/May sweep to my revenge." At this point, Hamlet is completely justified in his feelings, and most would agree that his revenge is morally right. Although the act of murder itself is wrong, an "eye for an eye" almost wholly justifies it. The gravity of Claudius' crime grows when one considers that all the deaths throughout the play would not have come if it were not the murder. The crime itself is, in a sense, worse because of the circumstances; not a simple murder, but the murder of one's brother wholly for personal gain, his crown and queen. It is this which balances out any morally wrong actions Hamlet may take. Hamlet, on the other hand, begins the play as a very rational and intelligent man. Although it is shown he can be impulsive and rash, his rationality wins out - at least in the beginning of the play. When seeing his father's ghost, he unquestionably accepts all he hears as truth, but doesn't act on it until he can verify it in some way.

Confirmation Bias Essay

Confirmation bias can influence perceptions is about not only what has already occurred but also our predictions of what yet occur. Confirmation bias can generate their own confirmations, some of these perceptions and predictions (Myers, 2012, p. 72). The phenomenon of confirmation bias starts with overconfidence, which can originate in a lack of knowledge regarding a topic or task. It I was to ask someone that is overconfident if they were good at some task or operation in which they no experience or expertise, they would answer with a resounding affirmation that they would be good at any task they do. However, this overconfidence may only be born of an ignorance of what the task or operation really requires to be performed successfully. Sometimes it is our memories that lead us astray into a false confidence. Remembering times when we were â€Å"almost† right perhaps (Myers, 2012, p. 75). We can often remember what we want to remember rather than what actually occurred. We often hear what we wanted to hear. Sometimes we even tell ourselves there were reasons for some of our less conscious actions. Intellectual deceit is a phenomenon that fortifies and cultivates the occurrence of confirmation bias. Myers (2012) states â€Å"The â€Å"intellectual conceit† evident in judgments of past knowledge (â€Å"I knew it all along†) extends to estimates of current knowledge and predictions of future behavior. We know we’ve messed up in the past. But we have more positive expectations for our future performance in meeting deadlines, managing relationships, following an exercise routine, and so forth (p. 72). Another statement by Myers (2012) is â€Å"The construction of positive memories brightens our recollections† (p. 77). I believe this tendency helps us not only to develop enough intellectual deceit to become overconfident which leads to bias, but also this inherent psychosocial, psychological trait or habit causes us to recall our previous thoughts, intentions, and actions in  such a way that sheds favorable enough light on them for us to then perceive if not generate some sort of confirmation of our bias.

Inflation

There are various ways for the employees to voice out their dissatisfaction_ One of the ways is by using picketing. Under section 40 of Industrial Relation Act allow workers to attend at or near their workplace when they have a trade dispute. The purpose of picketing are communicate issues to the public and members the employers. Picketing also held at lunch time, and before or after working hours. Picketing is enforceable by law as long as it must not intimidate anyone. Must not obstruct the entrance or exit the organization. 3. Summarization National Union of Bank employees (NUMB) held another picket on the issue for discriminating its Muslim employees and also In support with the 27 employees that have been sacked by the bank. The picket was held at Clan Tuna Appear. The Muslim employees had been discriminated by disallowed them from performing their prayers in the office by the management. They had been sacked because they did not agree to transferred to other branches. They had been discussed with the upper management but no action taken by the authorities to settle the Issues.They will intone to fight for the workers until Hong Leone Bank reinstate the sacked 27 employees. The Mole when contacted with Shirring Oarsman officer of Hong Leone Bank stated that the fact of disallowed Muslim employees was untrue statement. They are not allowed because sugar Is located In a secured area as they were not the staff of the branches and they just only the NUMB members. No one is allowed to entered into a secured area as it has been a standard security policy of any financial institution.She also stated that its staff had been allowed to perform their prayers thou any prejudice. 33 Mall Issues The Issues arises Is discriminates Its Muslim employees by disallowed them to perform their prayers at Sugar. As the officer offing Leone state that the Muslim employees is not the staff of Hong Leone Bank. Many negotiations had been taken by the employees but all the negotiati ons is not success because there Is no action taken by them. The Muslim officials In the Human Resource Ministry,the Government and the authorities failed to protect Islam and Muslim employees.Besides that, 27 employees in the Hong Leone Bank were sacked because they did to agree to be transferred to other branches. 3. 4 Background Issue Among 300 of NUMB members held a picket at Clan Tuna Appear regarding to the Issues of discriminating Muslim employees by disallowed them to perform their Solar at Sugar and over 27 employees were sacked. This is because Sugar is located at the secured area. It is a security standard policy no one can entered into that area except for the staff of Hong Leone Bank. The officer of Hong Leone Bank state that they are not the Hong Leone Bank but they are NUMB members.Abdul Jamie as Vice President f Numb members reiterated the statement that Numb members are also the Hong Leone Employees. During the time of 2 December until 18 December they are still und er Hong Leone employment. NUMB General Secretary enforce Balance Ministry to take legal action for these discrimination. Hong Leone Bank cannot said that NUMB members is not Hong Lemon's employees. Besides that, over 27 employees were 1 OFF discussions were held but none of the ministry take action over these issues. Tan stated that the transfer is necessary to increase the effectiveness and efficiency ofHong Leone Bank. 3. 5 Analysis Many of the Numb members held a pickets to ensure that their dissatisfaction can being heard by the public. Among the issues arises is the discrimination towards Muslim employees. Muslim employees had been disallowed to perform their prayer at the Sugar. Department of Labor will remind the employers the right of Muslim employees. Besides that over 27 employees were sacked. Their termination is not valid without a valid reasons as they disagree to transfer to other branches and then they were sacked.For the dismissal of the contract the employers should at least give notice of termination. Under section 12 of Employment Act 1955 state that, Either party to a contract of service may at any time give to the other party notice of his intention to terminate such contract of service. In the Industrial Relation Act section 20 provides machinery for an employers to dismissed its employees. The employees can make claims if they believe their termination is without a valid reasons.If the Court agree that their termination is without a valid reasons under section 14 of the Employment act 1955,then the Court will reinstated their termination. 3. 6 Recommendation The company may used Conciliation to solve their problems. By using third party as a conciliator. Conciliator is one of the officer of Department of Industrial Relation. The conciliator will meet the both parties whether separately or Jointly. The conciliator will briefing the problems and help the parties to achieves mutual agreement. This way can be used if the negotiations is not useful.The conciliator will give the recommendations and advice. 3. Conclusion Discrimination on the Muslim employees should be stopped by the government. Under article 11 of the Federal Constitution state that every person have the right to practice their own religion and to propagate it. The employer has no right to obstruct Muslim employees to perform their prayers. Regarding the issues of termination without valid reason is void. As it contravene with the Employment Act. The employer must give a notice of a services before dismiss their employees. If the issues can be settled it would create harmonious work environment. Inflation The cost of inflation depends on two cases; perfectly anticipated inflation and unanticipated inflation. Anticipated inflation is defined when individuals are able to make accurate predictions of inflation, they can take steps to protect themselves from its effects. Also, trade unions might use their bargaining power to negotiate for increases in money wages to protect the real wages of union members. Households may want to switch savings into accounts offering a higher rate of interest or into other financial assets where capital gains might exceed price inflation.Businesses can adjust their prices and lenders can adjust interest rates. However, businesses may also seek to hedge against future price movements by transacting in â€Å"forward markets†. For example, many airlines such as Bahamas-Air may buy their fuel months in advance as a protection or ‘hedge' against fluctuations in world oil prices. In Contrast, When inflation is unanticipated, individuals do not reali ze that they should protect their real purchasing power against a rising price level until the price level has already risen and their real purchasing power has already fallen.In this instance, there will be gainers and losers, in terms of purchasing power, from the inflation. In general, unanticipated inflation causes a misallocation of resources. Firms, unions, banks, will push prices and wages up. Those who can do it best will cause a misallocation of resources. For example suppose workers at manufacturing companies in the Bahamas wage increases, and public employees don't. Then, resources (labor) will be reallocated due to the relative market power of the different workers.But more importantly, lenders such as Banks will lose with respect to borrowers, giving individuals an incentive to borrow. For instance, when Bahamian people take out mortgages in order to buy houses at fixed interest rates, they end up aying back less in real terms than they had contracted for, wealth is the n redistributed from banks and other financial institutions to homeowners with mortgages. In relative terms, borrowing becomes cheaper than paying in cash.Another example would be if individuals who retire on pensions that are fixed in nominal amount will find the values of those pensions in terms of the goods they buy eroded as years pass, in this case the redistribution is from pensioners to the owners of insurance companies and other financial institutions that have contracted to pay them fixed dollar amounts. Alternatively, individuals have the incentive to spend now before the price level rises further.This however, will push prices up even faster, and may cause the inflation rate to accelerate and if uncertainty continue to increase, consumers and investors become less certain about the future, as prices rise in an unanticipated fashion. They may change their pattern of spending, and be less willing to undertake projects that take a long time to payoff. The Costs of Hyperinfla tion. Hyperinflation is define when the prices of most goods and services skyrocket, usually more than 50% a month. It usually starts when a country's Federal government begins printing money to pay for fiscal spending.As the money supply increases, prices creep up as in regular inflation. However, instead of tightening the money supply to lower inflation, the government keeps printing more money to pay for spending. Once consumers realize what is happening, they expect intlation. T causes them to buy more now to avoid paying a higher price later. This boosts demand, causing inflation to spiral out of control. The only winners in hyperinflation are those who borrowed before the hyperinflation. Fortunately, the Bahamas have ever experienced hyperinflation but other countries such as Germany and Zimbabwe has.But if the Bahamas was to experience hyperinflation like Germany, the consumers can expect 1 . Inflation Expectations and Wage Demands: Price increases lead to higher wage demands as people try to maintain their real living standards. This process is known as a Wage-price spiral'. 2. Arbitrary Re-Distributions of Income: Inflation tends to hurt people in Jobs with poor bargaining positions in the labour market – for example people in low paid Jobs with little or no trade union protection may see the real value of their pay fall.Inflation can also favour borrowers at the expense of savers as inflation erodes the real value of existing debts. 3. Business Planning and Investment: Inflation can disrupt business planning. Budgeting becomes difficult because of the uncertainty created by rising inflation of both prices and costs – and this may reduce planned investment spending. 4. Competitiveness and Unemployment: Inflation is a possible cause of higher unemployment in the medium term if one country experiences a much higher rate of inflation than another, leading to a loss of international competitiveness and a subsequent worsening of their trade p erformance.

Worlds Worst Dictators Essay Example

Worlds Worst Dictators Essay Example Worlds Worst Dictators Paper Worlds Worst Dictators Paper World’s Worst Dictators There are many different dictators in the world, who have all done horrific and immoral things. They all have their worst points, and different people rank the â€Å"Worlds Worst Dictators† differently. I believe that Hu Jintao, from China, is the number one worst dictator, King Abdullah from, Saudi Arabia, is second, and Sayyid Ali Khamernei, from Iran, is third. Sayyid Ali Khamernei has been the dictator of Iran since 1989. Since then he has oppressed the rights of women, stored nuclear weapons, carried out public hangings, and destroyed media and music stores. Sayyid Ali Khamernei is number three on our list of World’s Worst Dictators because he has executed hundreds of people, supports nuclear activity in his country, even though the UN has restricted all nuclear weapons. King Abdullah has been the dictator of Saudi Arabia since 1995. He has led a punitive justice system, where he can sentence anyone including teens to death for little or no reason. One of the worst things about his rule is that women aren’t allowed to do anything without the permission of a male guardian. He has been suspected to be involved in the 9/11 attack, because many of the bombers were Saudi, but no concrete evidence has been found. King Abdullah is number two on out list. Hu Jintao of China has been in power since 2002. He has China locked down and ALL public news formats are censored and restricted by the Chinese government. Hu Jintao supports the â€Å"one child policy† which limits all the families in China to only having one child. In our opinion the worst thing that he has done is forced abortions because of the one child law. He enforces that law brutally and forces mothers who already have a child to abort their pregnancy. That is why we believe that Hu Jintao is the number one World’s Worst Dictator. All of these people have been dictators for a long time and have done many horrific things. Sayyid Ali Khamernei has carried out public hangings, King Abdullah sentences people to death at will, and finally Hu Jintao forces abortions. Each one has ruled cruelly and has violated the moral principals that hold the world together.

Best Podcasts for Law Students

Best Podcasts for Law Students Blogs can be helpful for new law students, but many people enjoy listening to podcasts as well. Podcasts can be a great way to get information and give your very tired eyes a break from reading online. To help you update your podcast subscriptions, here is a list of some of the best podcasts for law students. Best Law Podcasts Enchanting Lawyer Podcast: This podcast is hosted by Jacob Sapochnick who runs his own solo practice and focuses on helping lawyers understand how to run and grow a business. Tips will be shared for using social media to grow your business and general marketing tips. Gen Why Lawyer Podcast: This weekly podcast is hosted by Nicole Abboud who interviews Gen Y attorneys who are accomplishing great things in their legal careers. She also talks to non-practicing attorneys who are using their legal knowledge to explore other ventures. Law School Toolbox Podcast: The Law School Toolbox podcast is an engaging show for law students about law school, the bar exam, legal careers, and life. Your hosts Alison Monahan and Lee Burgess offer practical tips and advice on academic matters, careers, and more. You might not always agree with them, but you won’t be bored listening. The goal is to impart useful, actionable advice in an entertaining manner. Lawpreneur Radio: This podcast is hosted by  Miranda McCroskey  who hung out her shingle over ten years ago to found her own firm. Her goal is to create a community where members are both lawpreneurs who’ve figured out how to successfully start their own firm and the vendors who support them. If you are ever thinking of hanging out your own shingle, check this out. Lawyerist Podcast: The  Lawyerist  is a popular legal blog and is also a podcast. In this weekly podcast, the hosts Sam Glover and Aaron Street chat with lawyers and interesting people about innovative business models, legal technology, marketing, ethics, starting a law firm, and much more.​ Legal Toolkit Podcast: This podcast is a  comprehensive resource for professionals in law practice management. Your hosts Heidi Alexander and Jared Correia invite forward-thinking lawyers to discuss the services, ideas, and programs that have improved their practices. Legal Talk Network:  The Legal Talk Network is an online media network for legal professionals that produces a large number of podcasts on a variety of different legal topics. The programs are available on-demand through various channels, including on the Legal Talk Network website, iTunes, and iHeartRadio. The flagship show called  Lawyer 2 Lawyer  has over 500 shows for you to listen to and download. If you are looking for a podcast to fill some extra commute or downtime, this may be the one for you. Resilient Lawyer: This podcast is hosted by Jeena Cho who offers mindfulness training for lawyers and is the author of The Anxious Lawyer. Jeena interviews a number of attorneys who share their stories about practicing law and finding a path to happiness. Thinking Like a Lawyer: This podcast is brought to you by the folks at Above the Law. Your hosts are Elie Mystal and Joe Patrice. They discuss a variety of topics, promising an entertaining and fun listen for those interesting in talking about the world through a legal lens.

Research Paper on Jimi Hendrix Term Example | Topics and Well Written Essays - 1000 words

Research on Jimi Hendrix - Term Paper Example Discography is about examining the information associated with recording sounds performed by an artist within a specified musical genre. In line with this, Shuker revealed that discography requires the gathering of music information such as the name of the artist(s) involved in the entire music recording process, the time and place where the music recording process took place, the title of the musical piece being performed by the musicians, the exact date when the music was publicly released, and the success rate of the musical piece which can be noted down through the actual sales figures and its actual chart position in the market (Shuker 80). Among the five famous musical recordings of Jimi Hendrix includes: The Cry of Love (1971), the Rainbow Bridge (1971), Crash Landing (1975), Radio One (1988), and Hendrix in the West (1972). Specifically the Cry of Love, the Rainbow Bridge, Crash Landing, and Radio One were studio albums whereas Hendrix in the West was one of the most successful live albums of Hendrix (Gelfand 92 - 101). Back in April 1970, the Cry of Love 31-city tour was launched at LA Forum housing a total of 20,000 audiences (Gelfand 92). Basically, the city tour highlighted the presence of the new Experience line-up together with the Mile’s band and the Buddy Miles Express as part of its opening act (ibid). Right after the city tour, Hendrix intentionally killed himself with drug overdose (Gelfand 100). Half a year after his death, Hendrix’s last 10-song studio album which was entitled â€Å"The Cry of Love† was released in the world market (Faralaco 110). In UK and U.S., this album was released between March 5 and 6 of 1971 which eventually became the top 2 and 3 in England and the U.S. charts respectively (Faralaco 91; Gelfand 101). The Cry of Love was released in LP format. Lasting for 39 weeks, the sales of this album reached gold and platinum award in UK and US respectively (RIAA a). Even though Hendrix died at the age